Kevin Lisbie struck a first-half winner as Colchester saw off Bristol Rovers with a 1-0 win at the Weston Homes Community Stadium.
Lisbie, on loan from Ipswich Town, fired home sweetly from 15 yards on the half-hour mark after linking up well with Phil Ifil.
Colchester enjoyed the better of the first half, with Rovers goalkeeper Mikkel Andersen saving Magnus Okuonghae's near-post header, while Anthony Wordsworth went close.
Lisbie then squandered a gilt-edged chance, hitting thin air rather than the ball when through on goal.
But five minutes later, Lisbie made amends, tapping home his goal, before Andersen came out on top following a one-on-one with Wordsworth.
Rovers' only meaningful first-half effort came when Chris Lines' effort was saved well by United keeper Ben Williams from point-blank range.
The second half saw less in the way of clearcut chances but Colchester again enjoyed more of the possession.
Andersen denied both Ifil and Marc Tierney but in the end, a single goal proved enough for the high-flying Us.
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