Blue Square Premier strugglers Barrow landed a lucrative FA Cup third round trip to Sunderland after making short work of their league's runaway leaders Oxford at Holker Street.
Phil Bolland, Carlos Logan and Marc Goodfellow all scored for the home side before the visitors - five points clear at the top of the table - responded with a last-minute consolation from James Constable.
Barrow's win will send them to the Stadium of Light on January 2 fresh with the memory of last season's third round trip to Middlesbrough, when the non-leaguers acquitted themselves well in a 2-1 defeat.
Blue Square rivals Luton wrapped up what technically amounted to a giant-killing as they swept aside League Two high-flyers Rotherham at Kenilworth Road.
Adam Newton set the home side on their way in the seventh minute and further goals from Alan White (19) and Claude Gnakpa (68) earned the Hatters a third round trip to Southampton.
Goals from Ian Thomas-Moore and Terry Gornell won Tranmere a third round home tie with Wolves after a 2-1 win at Aldershot, whose consolation came late from Oliver Bozanic.
Robert Grant scored the only goal of the game as Accrington Stanley edged to a 1-0 win over Barnet at Underhill and Stanley will now entertain Gillingham at the next stage.
Leeds will meet Manchester United after a 5-1 extra-time win over non-League Kettering.
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