MK Dons midfielder Peter Leven is a doubt to face Tranmere.
Leven lasted just 36 minutes of the Johnstone's Paint Trophy defeat to Southampton on Wednesday night before coming off with a groin injury and he is unlikely to face Rovers.
On-loan Arsenal midfielder Mark Randall replaced Leven against the Saints and could make his first start for the Dons while fellow loanee Andros Townsend is also in contention.
Townsend was cup-tied on Wednesday night after representing Leyton Orient in an earlier round but is available as Paul Ince's side look to win to keep pace with the play-off pack.
Defender Sean O'Hanlon remains a long-term absentee as he recovers from knee surgery and he could be fit by March.
Tranmere have signed Northampton defender Chris McCready on loan for the rest of the season.
McCready, who played for Tranmere earlier in his career, has joined the Prenton Park club until May after losing his place at Sixfields following the return to form of Craig Hinton.
Rovers boss Les Parry, whose only injury absentee is striker Bas Savage (Achilles), is delighted to have signed McCready and is now trying to add a striker.
He said: "We are keeping clean sheets and fortunately our injury record to date has been unbelievable, but that might not carry on.
"It's an area of the field we needed strengthening in. Now that is put to bed everything is focussed on finding a striker."
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