Blue Square Bet Premier League leaders Wrexham sprung the biggest surprise of the FA Cup second round by knocking out League One Brentford.
Midfielder Jamie Tolley fired the Welsh side in front with a volley from the edge of the box after half an hour at Griffin Park and the Dragons held on to get their name in the hat for the third round.
Wrexham boss Andy Morrell said: "I'm delighted for every one of the players. We couldn't even fill the bench today because we had another game going on at home in the Welsh Cup but we thoroughly deserved today I thought."
Fellow Blue Square Bet Premier club Tamworth also made it through as they beat league rivals Gateshead 2-1.
Redbridge were the lowest ranked side left in the competition but they were thumped 5-0 by League Two high fliers Crawley Town, as Matt Tubbs helped himself to a hat-trick and Kyle McFadzean and Andy Drury completed the rout.
MK Dons progressed by beating Barnet 3-1 despite Izale Mcleod's goal against his former club, and a much changed Charlton team overcame Carlisle 2-0 at The Valley with goals from Michael Morrison and Jason Euell for the League One leaders.
Bradford City are in the third round for the first time in seven years after they knocked out AFC Wimbledon 3-1 at Valley Parade and Swindon Town, also of League Two, eliminated League One strugglers Colchester United after a stunning strike from Matt Ritchie secured a 1-0 win.
They will be joined by Gillingham who also took out opposition in the division higher than them, winning by the same score at Leyton Orient thanks to Curtis Weston's goal.
Cheltenham avoided a potential banana skin by beating Luton 4-2 in a pulsating game where Darryl Duffy managed to score and get himself sent off , while Bristol Rovers hammered AFC Totton 6-1.
Notts County booked their place in the third round after a Jeff Hughes double was enough to see off Sutton United 2-0, and both Sheffield sides also made it through as the Blades beat Torquay 3-2, while Wednesday overcame Aldershot 1-0.
Shrewsbury came from behind to beat Rotherham 2-1 including a goal from former Millers captain Ian Sharps, and Stevenage ruthlessly dumped out Stourbridge 3-0.
There are also ten sides who will have to replay for the right to take part in the third round. Non-league Chelmsford held Macclesfield 1-1 and will head to Moss Rose for a second game whilst Fleetwood scored twice in the last ten minutes, including a penalty from player-coach Jamie Milligan, to peg back Yeovil to 2-2.
Dagenham and Redbridge and Walsall will also need to play again after a 1-1 draw, whilst Southend and Oldham played out the same score, but neither Salisbury or Grimsby could break the deadlock in their tie, and so will be forced to meet again.
The highlights of the third round draw are a mouth watering Manchester derby, Tottenham hosting Chelteham and Southend or Oldham heading to Anfield to take on Liverpool.