Terry was on the bench as the Stamford Bridge club eased into the FA Cup quarter-finals with a 2-0 win at Birmingham City in their fifth round replay.
The defender has been out for the past few weeks and underwent knee surgery just a fortnight ago and Di Matteo was hopeful the Blues will be able to benefit soon as they try to get their season back on track following the sacking of Andre Villas-Boas.
“John has done very well and is determined to help the club, and we're pleased to see him. He's available,” he said.
“We have to take him with small steps because he's done well to come back in just over two weeks. But it was good to see him.”
The former England captain will be keen to get back to playing football after a controversial few weeks, where his stripping of the national team captaincy led to the resignation of then manager Fabio Capello.
The former England boss is one of those who have been named as a possible successor to Villas-Boas, but current temporary incumbent Di Matteo is refusing to be drawn on the question of whether he wants the job permanently.
“Would I take it? I'm very tired tonight. I have to think about Stoke City from an hour's time onwards, and that's all I'm thinking about,” said the former Chelsea midfielder
“Think whatever you want to think. I'm not thinking about anything else.”
The clash with Stoke is becoming ever more important as the loss to West Brom last week, which resulted in Villas-Boas’ sacking, meant the Blues fell three points behind fourth place rivals Arsenal in the Premier League.