UEFA Champions League
Wednesday, March 14. Kick-off: 1945
Juan Mata and Daniel Sturridge are set to be restored to the starting line-up, having both been substitutes during Chelsea's weekend win over Stoke.
Didier Drogba scored the winner at Stamford Bridge on Saturday and is likely to be preferred to Fernando Torres as Chelsea look to overturn a 3-1 deficit.
Meanwhile, Napoli's star man Edison Cavani is in prime condition for tonight's clash, having been left out of the 6-3 rout of Cagliari.
Ezequiel Lavezzi scored in Friday's victory - and netted twice against Chelsea - and will partner Cavani in attack.
Di Matteo excited about Napoli visit
Roberto di Matteo says he is excited by the prospect of facing Napoli on Wednesday evening, but has stressed the need for a 'great' performance.
Chelsea trail the Champions League encounter 3-1 from the first leg, and Napoli are firm favourites to progress to the last eight of the competition.
Walter Mazzarri's side travel to Stamford Bridge off the back of a 6-3 Serie A win over Cagliari on Friday night, while Chelsea narrowly saw off Stoke at the weekend.
Although the Blues have recorded two wins since Di Matteo took charge, they have still yet to move on from the departure of Andre Villas-Boas little over a week ago.
And Di Matteo realises tonight's encounter could be the toughest he faces during his short stint as the Chelsea manager.
"I am excited about the game, we have a big job on our hands [against Napoli] but the squad is in good shape and everybody is available so we are looking confident for tomorrow night," he told chelseafc.com.
"We want to finish the season in the best way and reach our objectives - we started well and the more you win the more confidence you can grow, which is positive in light of the match.
He added: "In a cup competition you need to deserve the little bit of luck that makes you go through.
"Personally it would be a proud moment, but more than anything we just want to do well for the club and our supporters and want the team to show a passionate performance and try to turn this tie around."