Chelsea trio Raul Meireles, Ramires and Branislav Ivanovic will all miss the Champions League final after FIFPro's attempt to appeal the bans was turned down.
The worldwide players' union had appealed to UEFA for clemency to enable the three Blues players, plus David Alaba, Holger Badstuber and Luiz Gustavo of Bayern Munich, to play in the showpiece final on 19 May.
However, UEFA rejected their request and even went as far as to say that the rules regarding suspension would not change for at least another three years.
A statement on FIFPro's website quoted Michael van Praag, a UEFA executive committee member who heads the Champions League rules group, and who considered the appeal.
According to van Praag: "We did not receive any request whatsoever concerning the yellow card rule, not even from the representatives of Bayern Munich. And so we will be continuing the rule for the next three years."
The yellow card rules for the Champions League differ from those used in other major international tournaments. Next month's UEFA European Championships in Poland and Ukraine will see yellow cards wiped out after the quarter final stage, giving players a clean slate for the latter knock-out rounds.
FIFPro had said it felt the punishment of a ban for the final after picking up a yellow card was "disproportionate" to the offence. However, van Praag indicated that the alternative rules would remain in place for the coming years in the competition
"Different rules can apply in different competitions," said van Praag. "The current rules are the result of a careful, democratic procedure."