Wigan Warriors captain Sean O'Loughlin will miss England's International Origin match against the Exiles on Friday after suffering a knee injury reports the BBC.
O'Loughlin missed Wigan's most recent game, a 33-32 victory over the Widnes Vikings last Friday because of the same problem.
It means the 30-year-old will miss the annual fixture, which sees England take on a team made up of foreign Super League players.
England coach Steve McNamara will not call up a replacement for the mid-season match in Warrington, despite being already without Leeds Rhinos trio Carl Ablett, Danny McGuire and centre Kallum Watkins.
There are other injury concerns for McNamara as Wigan hooker Michael McIllorum and Hull FC second-row Danny Tickle have also withdrawn from the squad.
England are yet to have won an International Origin series, being defeated in the only game of the 2011 series, while the 2012 series was tied 1-1.
This year, the series goes back to consisting of only one match.
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