Tottenham star Gareth Bale has been ruled out of his side's opening match against Premier League new-boys Crystal Palace.
Bale - who has reportedly told friends he will not play for Tottenham again - is said to be suffering with a foot injury.
The 24-year-old missed Wales' recent friendly match against the Republic of Ireland with the same problem.
His admission from Tottenham's squad to play the Eagles will add more fuel to speculation linking him with a world record move to Real Madrid.
When asked if Bale would be playing against Ian Holloway's Crystal Palace side, Villas-Boas said: "No, because he's been out injured for most of pre-season.
"He was involved in the games against Brentford and Swindon but not any others, for different reasons; pulled a muscle in his gluteus that prevented him from being involved for two weeks and he's been complaining of pain in the foot.
"I think the most natural thing is to see him not involved against Palace."
Villas-Boas dismissed suggestions Tottenham would be willing to cash in on the Welshman if a bid in excess of £80million was to come in for their star asset.
"We've been very, very clear with everybody regarding the player and our willingness to hold on to him," added the Portuguese tactician.
"Obviously in football anything can happen in the end but this is a player we cherish a lot and has helped us progress and ideally we want to continue with him."
Villas-Boas will be hoping Bale will be back for Tottenham's first home game of the season against Swansea on August 25.