Liverpool captain Steven Gerrard and Tottenham defender Kyle Walker have been ruled out of England's friendly encounter against Chile on Friday.
Both players sat out of training this morning, while Chelsea midfielder Frank Lampard took part but is struggling with a cold.
Roy Hodgson, who confirmed Gerrard and Walker's absence this morning, has seen his England squad be decimated by injury in the lead up to the first of their final two games of 2013.
Manchester United duo Danny Welbeck and Michael Carrick have already withdrawn, with the latter set to miss up to six weeks with an Achilles injury.
Gerrard's Liverpool team-mate Daniel Sturridge is also struggling to be fit in time for the game at Wembley.
Liverpool boss Brendan Rodgers told the Guardian on Saturday: "It is something they are going to have a look at. Daniel hurt his foot and I just have to see how he is with the medical guys. Steven was a wee bit stiff around his hip. Again, he needs to be assessed."
Arsenal boss Arsene Wenger was spotted at the training session - taken at Arsenal's London Colney training ground this morning because of a bug sweeping their usual base, St George’s Park - amidst reports wants Jack Wilshere to play in the game against Chile, and not against Germany next week.
According to reports Wenger is anxious to ensure that Wilshere is not over-worked as he continues to nurse an ankle problem, and that England will leave him out for the showdown against Joachim Low's side on Tuesday.
Hodgson initially named a 28-man squad for the games against Chile and Germany, with Southampton pair Rickie Lambert and Adam Lallana expected to start against the South Americans