Former Wales captain Ryan Jones had been ruled out of the Six Nations tournament after sustaining a hamstring injury, reports BBC Sport.
The 32-year-old captained Wales to victory at last year's tournament as the Welsh side retained their crown but the Osprey's back-rower was injured in Friday's Heineken cup 36-3 defeat to Leinster and will now be replaced by team-mate James King.
Warren Gatland is back in charge of Wales after a successful tour of Australia with the British and Irish Lions and has a few injury concerns heading into the tournament next month.
Hooker Richard Hibbard is top of this list but the 50-year-old has called up Newport Gwent Dragons hooker Sam Parry and Scarlets second-row Jake Ball as cover.
Gatland also named Sam Warburton in his 32-man squad last week and it was reported yesterday that the flanker would be fit to play the first game against Italy on February 8.
Write for GiveMeSport! Sign-up to the GMS Writing Academy here: http://gms.to/1a2u3KU