Andy Murray is out of this year's Aegon Championships after suffering a straight sets defeat to Radek Stepanek in the third round.
The number three seed was unable to take his chances in the opening set, and went on to lose 7-6(10), 6-2 on the centre court at Queen's in west London.
Having received a bye in the first round following his run to the semi-finals at the French Open, it means Murray has only won one match on grass heading towards the defence of his Wimbledon title at the end of the month.
Victory over Paul-Henri Mathieu was relatively straightforward yesterday (Wednesday), but Stepanek showed his years of experience to hang tough when it mattered - particularly in the first set.
The 35-year-old saved eight set points in the tie break, and also fought back in the earlier exchanges after losing his serve in the opening game of the match. After claiming the opening set in just over an hour, the second proved much more routine.
Defeat ends Murray's 19-game winning streak on the lawns, having won this event last year as well as the major at SW19 a few weeks later. This loss seriously hampers his progress however, with the Scot's next match scheduled to be in the first round of that Grand Slam in 11 days.
That will give new coach Amelie Mauresmo plenty of time to try and work on some things after her appointment on Sunday, and the world number five is unlikely to be panicking ahead of his return to Centre Court.