Southampton suffered defeat against Swansea at St. Mary's after Jonjo Shelvey's second half strike beat Fraser Forster and flew into the top corner.
The Saints are aiming to finish in the Champions League places and with the teams below them winning and the two above drawing, it seemed like the perfect opportunity to make up ground.
It was not to be though and after James Ward-Prowse saw his early effort saved by Lukasz Fabianski, Shelvey's long-range strike was enough to seal the points for the Welsh side.
Early chance missed
The best chance of the first half fell to the home side who dominated possession in the opening 45 minutes.
A neat move down the right saw Nathaniel Clyne pull the ball back to James Ward-Prowse. The young midfielder's strike found the target but was kept out from close range by the feet of Lukasz Fabianski.
Article continues below
Despite their superiority in the game, Ronald Koeman will be disappointed that his side did not create many chances which would come back to haunt them in the second half.
As the second half continued the Swans grew into the game and after Shelvey sent out the warning signs with a rasping shot that hit the post, he made no mistake second time around.
The former Liverpool man picked the ball up and shifted the ball to his right before firing an arrowed strike into the top right-hand corner.
Fraser Forster got a hand to the shot and may have done better but the away side led with key men Gylfi Sigurdsson, Wayne Routledge, Jordi Amat and Ki Sung-Yeung all missing.
No way back
The home side began the search for an equaliser with seven minutes remaining although Swansea had looked comfortable all afternoon and Fabianski was rarely troubled.
Sadio Mane had two efforts cleared off the line before Ryan Bertrand was shown the red card in the closing minutes of the match after a heavy tackle on Swansea substitute Modou Barrow.
The last chance of the afternoon fell to Graziano Pelle who volleyed well over the bar from 12 yards giving Swansea their first victory at Southampton for 62 years and condemning the hosts to their first defeat in seven league matches.
Southampton player ratings
Forster - 6
Clyne - 7
Fonte - 7
Yoshida - 6
Bertrand - 6
Reed - 7
Davis - 7
Ward-Prowse - 6
Tadic - 5
Elia - 7
Pelle - 6
Mane - 6
Seager - NA