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England captain Wayne Rooney is set to miss England's Euro 2016 qualifier against Estonia through injury.
For the third day in succession the 29-year-old forward was unable to train with his international team-mates after an ankle complaint sustained in Manchester United's defeat to Arsenal.
Rooney is set to miss Friday's clash with Estonia as a result and is a major doubt for next week's trip to Lithuania in the Group E finale.
Regardless of the injury, the striker is expected to attend the final Wembley qualifier as he wants to pick up his commemorative golden boot for breaking Sir Bobby Charlton's scoring record.
Rooney last month broke the 45-year long record with a penalty against Switzerland and will receive the boot from the 1966 World Cup winner before kick-off.
Theo Walcott appears the most likely replacement up front for Roy Hodgson's men, especially as Danny Ings is also set to miss the Estonia encounter.
The Liverpool striker is in the England squad for the first time, but again sat out training at St George's Park on Thursday with an ankle complaint.
Michael Carrick is a doubt after missing the session with a knock, although Jonjo Shelvey returned to action.
Andros Townsend and Kyle Walker were involved, having been drafted into the squad following the withdrawal of John Stones earlier in the week.