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World Anti-Doping Agency (WADA) president Sir Craig Reedie said "the world is watching and we have acted" after the organisation decl ared Russian Anti-Doping Agency (RUSADA) non-compliant with international sport's anti-doping code.
Russia was provisionally suspended from all international athletics competitions by world governing body the IAAF last Friday after WADA's independent commission's findings of state-sponsored, systemic doping practices.
WADA's foundation board, which met at Colorado Springs in the United States on Wednesday, said RUSADA should be declared non-compliant immediately, with five other countries also given the same treatment.
Non-compliance means failing to respond to WADA's requests for information and as a consequence, organisations cannot operate until they become compliant.
Reedie said: "The message is clear - there will now be greater focus on strengthening compliance work so all anti-doping organisations worldwide are held accountable to deliver robust anti-doping programs.
"Our priority is now on ensuring all our partners are fully compliant and have watertight anti-doping systems that protect clean athletes and reassure sports fans worldwide.
"Make no mistake, we will not rush this process of compliance, we will do it right - the integrity of sport is under threat.
"Anti-doping in sport is under the spotlight today like never before, and WADA, along with our partners, have begun the work needed on the road to recovery for Russia. The world is watching and we have acted.
"We will conduct the necessary meetings with the Russian authorities in respect of the non-compliance status of RUSADA that tests athletes in all sports within Russia.
"A WADA expert team will then meet with the task of ensuring the continuation of testing in Russia. Any information brought forward to me as a result will allow me to make a considered decision on whether or not to extend the independent commission's mandate.
"The theme of the day has clearly been investigations. I will now write to all public authority stakeholders and ask them to make further contributions specifically to fund anti-doping investigations. Following any commitments made, I will then immediately approach the International Olympic Committee to seek matching funding."
The Russian Olympic Committee (ROC) has agreed to oversee changes and said it was "determined" clean athletes will compete at the Rio Games next summer and any drug cheats will be "punished".
The ROC has agreed to make RUSADA, the anti-doping laboratory in Moscow, and the National Athletics Federation (ARAF) "compliant with the WADA code and all other international anti-doping regulations as soon as possible" and said all athletes, officials and coaches found guilty of doping will be punished in accordance with international anti-doping regulations.
The WADA independent commission report which said Russia was guilty of state-sponsored doping also added that it was not the only country "facing the problem of orchestrated doping in sport."
Andorra, Argentina, Bolivia and Ukraine were also declared as non-compliant, with Brazil, Belgium, France, Greece, Mexico and Spain placed on a compliance watchlist.'
The pressure has also intensified on Kenya, who had earlier been warned by WADA that they could be ruled non-compliant if they failed to provide satisfactory answers to questions about their anti-doping programme.
The African nation topped the medal table at August's World Championships in Beijing, amid allegations of doping cover-ups in the country.
A statement from WADA posted on Twitter read: "WADA has requested Kenya to answer questions relating to its anti-doping program. Await those answers, and if unsatisfactory, then it could be a matter for Compliance Review Committee.
"WADA is encouraged by media reports that Kenyan cabinet has now approved rules and approved laws and that it will commit funding to Kenyan anti-doping program."
Athletics Kenya vice-president David Okeyo, an IAAF council member, has been referred to the IAAF ethics commission after it was revealed he was the subject of a police investigation in his native Kenya.
Okeyo is alleged to have siphoned off funds from a sponsorship deal between the national association and sportswear firm Nike.