Jamie Murray and Bruno Soares' winning grand slam run came to an end in the third round of the French Open.
The pair won the title in their maiden slam together in Australia but were unable to make it past 16th seeds Marcin Matkowski and Leander Paes in Paris.
Having won their eight previous slam matches, the British-Brazilian pair lost two tie-breaks, going down 7-6 (7/5) 7-6 (7/4).
Murray was looking to reach the quarter-finals for the first time at Roland Garros and he and Soares were the better team in the first set after retrieving an early break.
But they missed four further break points and were undone in the tie-break by veteran duo Matkowski and Paes, who have a combined age of 77.
The second set followed a similar pattern, although this time it was the Polish-Indian duo pushing the pace and they duly prevailed in another tie-break.
The defeat means Murray must wait for another chance to reclaim the world number one ranking he briefly held this spring, while his run of consecutive grand slam final appearances ends at three.