Ryan Bertrand was the only absentee as England trained for the first time since Roy Hodgson finalised his 23-man squad for Euro 2016.
The Southampton full-back was present at Watford's London Colney base but was receiving treatment for a knock, having also sat out training on Tuesday.
Bertrand is set to miss England's final preparation match against Portugal at Wembley on Thursday but his fitness is not thought to be a major concern.
He is expected to be back on duty when the team arrive in France and available for the first group game against Russia.
Jordan Henderson and Jack Wilshere, two men who have had question marks over their fitness after injury problems, were the first outfield players on the pitch, kicking a ball between them before being joined by their team-mates.
The pair's inclusion came at the expense of fellow midfielder Danny Drinkwater, who was cut alongside winger Andros Townsend on Tuesday.
Eighteen-year-old Marcus Rashford and injury-plagued Daniel Sturridge will both have felt fortunate to be present, with Hodgson having resolved to take all five of his striking options to France.