Plymouth skipper Carl Fletcher and fellow central midfielder Luke Summerfield are expected to be ruled out of the home Championship game against Barnsley.
Fletcher suffered a cut knee in the opening stages of Saturday's 2-0 home defeat by Blackpool and Summerfield, his replacement, then sustained a hamstring pull later in the match and had to be replaced himself after 73 minutes.
Summerfield's substitution led to an introduction - and debut - for 17-year-old right-back Ryan Leonard with Karl Duguid moving into midfield, a role he occupied last season.
Head coach Paul Mariner has since recalled midfielder Yoann Folly from his loan spell at Dagenham & Redbridge as cover for his injury-hit squad.
Central defender Kari Arnason (hip), on-loan Burnley full-back Richard Eckersley (ankle) and winger Craig Noone (knee) are also ruled out along with right-back David McNamee (hamstring). Winger Alan Judge, who was substituted on Saturday with a groin injury, should be fit but defender Chris Barker (hamstring) is rated 50-50.
The game had to be rescheduled after the initial clash had to be abandoned due to a waterlogged pitch on November 28 with Barnsley leading 4-1 with over half an hour left.
Barnsley midfielder Filipe Teixeira is a major doubt.
The Portuguese playmaker, on loan from West Brom, was forced out of Saturday's home defeat to Doncaster with an abdominal strain.
Manager Mark Robins also has doubts about defender Bobby Hassell, who sustained a knock in training on Friday and sat out Saturday's derby, and striker Daniel Bogdanovic, who has been struggling with an ankle injury.
Defender Rob Kozluk, sidelined since late January with a knee injury, has returned to full training and could be included but Robins has revealed he has only 18 fit players available.
Copyright (c) PA Sport 2009, All Rights Reserved.
Do YOU want to write for GiveMeSport? Get started today by signing-up and submitting an article HERE: https://www.givemesport.com/writeforgms