Mark Byrne is ruled out of Barnet's home meeting with Gillingham.
The on-loan Nottingham Forest midfielder came on as a substitute against Northampton at the weekend after recovering from a knee injury.
However, he had to come off again 15 minutes later after injuring his other knee in a collision with Cobblers goalkeeper Chris Dunn.
Rossi Jarvis or Clovis Kamdjo will be added to the squad in Byrne's absence.
Boss Mark Stimson is likely to name an unchanged starting line-up against his former club following Saturday's thumping 4-1 victory which lifted the Bees off the foot of the table.
Defenders Darren Dennehy and Daniel Leach (both foot), winger Glenn Poole (ankle) and striker Danny Kelly (hamstring) are still sidelined.
Gillingham boss Andy Hessenthaler is unlikely to risk defender Simon King.
King was on the bench after 18 months out with an ankle injury as the Gills won their first away game of the season at Oxford on Saturday.
But Hessenthaler will give him some reserve-team action before he gets any first-team minutes under his belt.
Garry Richards (toe), Andy Barcham (ankle) and Curtis Weston (thigh) are also unlikely to feature against the Bees but will figure in a specially arranged reserve game soon to gain match fitness.
Mark Bentley, Dean Rance (both metatarsal), Jack Payne (foot) and Mark McCammon (Achilles) all remain on the treatment table and Callum Davies will sit out the second game of a three-match ban.
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