Harlequin Ladies Football Club, also known as Harlequins Ladies, is a women's rugby union club based in Guildford, Surrey, England and in Isleworth, Middlesex, England. They were founded in 1995 as the women’s team of Harlequin FC and rebranded in 2017 after merging with Aylesford Bulls. In 2017, they were selected as one of the franchises for the new Premier 15s league but also cater for new, aspiring and social players within their 3rd XV squad.