Tranmere boss Les Parry will check on the fitness of goalkeeper Tony Warner and winger Andy Robinson ahead of the home clash with Bristol Rovers.
Both players picked up knocks during Saturday's 1-1 draw at Charlton and will be assessed closer to kick-off.
Warner sustained a thigh strain and Robinson picked up a groin injury, leaving Parry facing a nervous wait over their availability.
Ash Taylor also picked up a knock at The Valley but it is unlikely to rule the midfielder out the clash with Dave Penney's men.
However, highly-rated rookie striker Dale Jennings will miss out again with a groin injury.
Parry said: "He's the type of player who needs to feel he's right so we're going to rest him for a few days before we push him on again."
Bristol Rovers boss Dave Penney will be without defender Jerel Ifil.
The centre-back, who recently arrived at the club on a non-contract basis, was dismissed in Saturday's 2-0 defeat to fellow-strugglers Dagenham for a professional foul and will now serve a one-match ban.
Cian Bolger or James Tunnicliffe could replace the 28-year-old in the starting line-up.
Fellow defender Danny Coles is almost certain to miss out after being stretchered off during the second half of the weekend's defeat with an ankle injury, although the severity of the problem is still unknown.
Captain Stuart Campbell made his return from a hamstring problem against the Daggers and is expected to continue in midfield, while left-back Gary Sawyer could continue following his return from a thigh problem.
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