Barnsley have confirmed their initial approach to speak with Leyton Orient manager Russell Slade has been rejected.
The Tykes, in the hunt for a new manager following the departure of Mark Robins 11 days ago, have already failed in their attempt to land Rochdale boss Keith Hill.
A Barnsley club statement read: "Barnsley Football confirm they have made an approach to Leyton Orient to speak with their first-team manager, Russell Slade. Leyton Orient have refused our request at this time."
Orient chairman Barry Hearn insisted on Wednesday night that Slade, who steered the Londoners to within one point of the npower League One play-offs this season, had "shaken hands" on a new deal.
Barnsley's first-choice Hill held talks with the Oakwell hierarchy last week, but failed to agree terms with the South Yorkshire club.
Brian Laws, Kevin Blackwell, Danny Wilson, Phil Parkinson and Alan Irvine have also been linked with the role.
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