Trent Alexander-Arnold has been ruled out of the European Championships due to injury.
But he would not see out the full game as he sustained a quad injury with minutes to go in the game.
Alexander-Arnold looked close to tears as he was helped off the pitch.
Gareth Southgate admitted in his post-match interview that the injury did not look good.
"We've got to see, it's obviously not a good sign that he's had to walk off the pitch, and he was in some discomfort," he said, per Sky Sports.
"We're so close to the finals that for any player, injuries makes them a doubt. But we will just have to see over the next 48 hours."
It has now been confirmed that he will take no part in the upcoming tournament.
"Trent Alexander-Arnold is out of the Euros," the Athletic's James Pearce wrote.
"Devastating news for the Liverpool right-back. Scan revealed he suffered a grade two quad tear v Austria. Out for 4 to 6 weeks. Will return home from the England squad tonight."
It's such a shame that one of England's most talented players will not play in the tournament.
Alexander-Arnold tried so hard to force his way back into Gareth Southgate's plans, which makes his injury even more heartbreaking.
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England still have good cover at right-back, though, with Kyle Walker, Reece James and Kieran Trippier all in the squad.
Southgate will now call up an additional player to replace Alexander-Arnold.
Jesse Lingard, James Ward-Prowse, Ollie Watkins, Aaron Ramsdale, Ben Godfrey and Ben White were cut from England's squad earlier this week.
The FA have confirmed that Southgate will announce the Liverpool man's replacement after England's game against Romania on Sunday.