The advert showed highlights of Real Madrid’s dramatic 3-2 victory over Man City back in September 2012 - a pulsating Champions League encounter in which Cristiano Ronaldo scored the winning goal.
The advert shows Cristiano celebrating and then immediately cuts to Messi sitting at home watching the action from home with a bunch of people - supposedly his mates, but actually actors - while eating Lays crisps.
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It goes without saying that UEFA’s intention wasn’t to embarrass Messi, whose Barcelona side were knocked out of the Champions League by Atletico in the quarter-finals, but the fact he was sat at home watching Ronaldo score made some people on Twitter wonder.
Scroll down this page to see how Twitter reacted to the advert.
Video: UEFA's advert
City v Real Madrid was a damp squib
If Messi was at home watching the first leg of the semi-final between City and Real Madrid while eating crisps with strangers, then we feel a bit sorry for him.
The game was dreadful - one of the worst Champions League semi-finals in memory - and we can only hope the return leg at the Bernabeu next week is more entertaining.
Joe Hart produced a fine save from Pepe, Zinedine Zidane split his trousers for the second Champions League match in a row and that was about it.
Zidane hopes CR7 will be back for second leg
The absence of Cristiano Ronaldo didn’t help the spectacle and Zidane admitted after the match that the Portuguese superstar might not be fit for the second leg.
Karim Benzema could also miss the second leg, although Zidane hopes both stars will be fit for Real Madrid’s biggest match of the season so far.
"We hope that they can both be fit for the second leg, that's the idea," Zidane was quoted as saying by Sky Sports. "But we can't say right now. We have to see. We have to take it day by day and see how they are.
"Cristiano felt something after that last training session. That's why he didn't play. Karim, it was a little bit different. But at the end of the day, little by little, he felt a little bit worse in himself. We don't want to gamble with his fitness. We know how important both players are for us."
Have UEFA inadvertently trolled Lionel Messi with this advert? Have your say by leaving a comment below.