The Matchroom Boxing chief could barely keep a grin off his face as both men stared intensely at each other for several long seconds.
Joshua was the first to break his gaze, raising his fist above his head in salute, but Usyk kept on staring at him for a few moments before he turned to face the crowd.
WBA, IBF and WBO heavyweight champion Usyk puts his titles on the line against the British superstar on August 20 in Saudi Arabia at Jeddah’s Super Dome.
AJ, 32, will be hoping to avoid a repeat of their first encounter at the Tottenham Hotspur Stadium last September, where he lost his belts in convincing fashion in a rather one-sided affair.
However, he has vowed to put things right this time, having replaced former trainer Rob McCracken with well-respected coach Robert Garcia.
“God willing I’ll perform and I’ll become three-time heavyweight champion of the world,” he told the press conference.
WATCH: Anthony Joshua and Oleksandr Usyk share intense staredown ahead of their rematch
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“The great thing is I’ve got a second chance. What got me into boxing in the first place – when I was a youngster I got in a little bit of trouble every now and again and I was blessed with a second chance and I found boxing. And I took it with both hands.
“So if you know me and a lot of my story, you know I’m the comeback king. You can put me down. But it’s difficult to keep me down.
“Sometimes if you keep things simple you can achieve great things. Robert’s been great in the camp. Definitely the hunger’s still there.
“Blips happen, things happen in life. We’re still on the road, still on the road to undisputed for sure.”
Usyk, meanwhile, spoke only to send a message of support to his people in Ukraine.
“As we all know we are not in the best condition at the moment back at home but we are doing what we have to do,” the champion added.
“We are doing our job. Together with my team we are working hard to achieve our goals. I never made some very loud and bright speeches.
“All I did was I just worked hard in my training camp and in my gym.
“That’s what I’m going to do until the date of the fight and then I will enter the ring and will make you happy with my boxing.”